How to extract DNA Deoxyribonucleic acid From chicken liver
Note:
DNA is easily to be cut by physical instinct because of a long chain structure. Therefore, need to stir gently. Operate quickly! Because there is a DNA resolve enzyme in cells. Since there is a surface-active agent in liquid detergent, the detergent destroys cell membrane and protein.
Materials:
Chicken liver 50g, Ice cold pure ethanol 500ml, Acetorein ( Acetocarmine), Blender, Beaker (1000ml, 200ml), Glass bar, Cotton gauze, Ice
2mol/l NaCl solution (NaCl 60g, Water 500ml) Detergent solution (Liquid detergent 1push, Water 200ml)
Procedure:
- Blend frozen chicken liver in the blender for 3 minutes.
- Divide 30ml liver juice for each group.
- Add 30ml of 2mol/l NaCl solution and stir it gently.
- Boiling (3)liquid for 5minutes.
- Filtrating (4)liquid by cotton gauze immediately and cooling by ice.
- Pour ice cold pure ethanol (double the amount of (5)liquid) gently along the glass bar.
- After a few seconds, white fibers ( look like cotton ) appear in the ethanol layer.( It is DNA.)
- Stain the white fibers by acetorcein (acetocarmine).
Extraction of DNA From chicken liver